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王志林,教授
 

Email: wangzl@nju.edu.cn

Tel: 025-83686082

王志林 教授

Prof. Zhi-Lin Wang

2001-present                Professor, Nanjing University

2003-2004                    Senior visiting scholar in University of Illinois at Uabana-Champaign

1996-2000                    PhD in Inorganic Chemistry, Nanjing University

1983-2001                    Teaching Assistant (1983- 1989), Lecturer (1989- 1995) and Associate professor (1995-2001)

1979-1983                    B.Sc. in Chemistry, Nanjing University

                  王志林 教授、博士生导师。19832000年分别获南京大学化学系无机化学专业学士和博士学位。1983年至今在南京大学化学化工学院工作,长期从事生物无机化学和超分子化学的研究及无机化学的教学工作。历任助教(19831989年)、讲师(19891995年)、副教授(19952001年)和教授(2001年)。20031月至20041月在美国University of Illinois at Uabana-Champaign做高级访问学者。1998-2005年任南京大学化学化工学院副院长,2005 -2009年任南京大学教务处长,2009年至今任南京大学党委常委、组织部部长。兼任教育部化学专业指导委员会副主任委员。

Research Interests

Fluorescence sensors development and application

The development of highly selective and sensitive fluorescent sensors has received considerable attention owing to their utility in clarifying and analyzing the roles of ions and biomolecules in living systems. A series of water-soluble fluorescent sensors based on the pyridine-containing tetraazamicrocycle were designed and synthesized, which show high selectivity and sensitivity for ratiometric recognition Of Cu2+, Zn2+, Hg2+, pyrophosphate, calf thymus DNA and bovine serum albumin, respectively. Wherein, an NBD-armed tetraazamacrocyclic fluorescent probe was specifically localized in lysosomes and successfully applied to visualize Cu2+ as well as to monitor Cu2+ level change in the lysosomes of living cells. A Zn2+ tetraazamacrocycle complex bearing three naphthalene moieties recognizes binds and causes damage to DNA, and shows considerable cytotoxicity against human cancer cell lines with a different apoptotic pathway from that of cisplatin.

Nanobiosensors development and application

Functional nanomaterial is the most vibrant area of nanomaterial science, which has broad application prospects in biology, energy, environment, information, aerospace and other high-tech fields. We developed a series of novel molecularly imprinted polymer based on nanomaterials, which were able to selectively recognize hydroquinone, aspirin, dopamine and bovine hemoglobin, respectively. We also prepared a series of coordination polymer nanoparticles and put forward the growth mechanism of the nanomaterials. The fluorescence properties, antibacterial and anti-tumor activities of nanomaterials were studied systematically, and their derivative materials were served as energy storage materials of alithium ion battery and electrode materials of super capacitor.

The interactions between biomacromolecules and metal ions and organic small molecules

1Arsenic is a biologically active element and can be metabolized by arsenic (+3) methyltransferase(AS3MT) into methylated arsenics in most organisms. However the catalytic mechanism of AS3MT is not clear. Thus the mechanism of methylation, especially the factors affecting the methylation of iAs3+, such as reductant, should be studied further. Our group systematically studied the binding sites of inorganic arsenic and methyl donor SAM in human AS3MT and the effects of different factors on the enzyme activity. We used the rapid equilibrium kinetic model to study the reaction sequence of arsenic methylation and initially proposed catalytic mechanism of AS3MT. Arsenic has two sides, which can serve as a drug to treat acute promyelocytic leukemia. Now we are engaged in studying the effects of inorganic arsenic and its metabolites on the leukemia in order to elucidate the mechanism of arsenic in the treatment of leukemia.

2) Hypoxia inducible factor 1 (HIF-1) is central to the hypoxic response in mammals. HIF-1α prolyl hydroxylase 3 (PHD3) degrades HIF through the hydroxylation of HIF-1α. Inhibition of PHD3 activity is crucial for up-regulating HIF-1α levels, thereby acting as HIF-dependent diseases therapy. We have designed and synthesized a series of ploynitrogen compounds and proline analogs, then studied their interactions with praline hydroxylase and their effects on the regulation of HIF-specific signal transduction pathways.

Selected Publications

1.   Indirect Transformation from Coordination-Polymer Particles into Magnetic Carbon-Coated Mn3O4 (Mn3O4@C) Nanowires for Supercapacitor Electrodes with Good Cycling Performance. Kuaibing Wang, Xiaoyan Ma, Zhiyang Zhang, Mingbo Zheng,Zhirong Geng, Zhilin Wang*. Chem. Eur. J. 2013, 19, 7084-7089.

2.   Residues in human arsenic (+3 oxidation state) methyltransferase forming potential hydrogen bond network around S-adenosylmethionine. Xiangli Li, Jing Cao, Shuping Wang, Zhirong Geng, Xiaoli Song, Xin Hu, Zhilin Wang*. Plos One 2013, DOI: 10.1371/journal.pone.0076709.

3.   Rapid-equilibrium kinetic analysis of arsenite methylation catalyzed by recombinant human arsenic (+3 oxidation state) methyltransferase (hAS3MT). Shuping Wang, Xiangli Li, Xiaoli Song, Zhirong Geng, Xin Hu, Zhilin Wang*. J. Biol. Chem. 2012, 287, 38790-38799.

4.   A potent antitumor Zn2+ tetraazamacrocycle complex targeting DNA: the fluorescent recognition, interaction and apoptosis studies. Jinghan Wen, Chengying Li, Zhirong Geng, Xiaoyan Ma, Zhilin Wang*. Chem. Commun. 2011, 47, 11330-11332. 

5.   Evidence for inhibition of HIF-1α prolyl hydroxylase 3 activity by four biologically active tetraazamacrocycles. Jing Cao, Zhirong Geng, Xiaoyan Ma, Jinghan Wen, Yuxin Yin, Zhilin Wang*. Org. Biomol. Chem. 2012, 10, 3913-3923.

6.   Preparation and Recognition Properties of Bovine Hemoglobin Magnetic Molecularly Imprinted Polymers. Xianwen Kan, Qun Zhao, Dalin Shao, Zhirong Geng, Zhilin Wang*, Jun-Jie Zhu. J. Phys. Chem. B 2010, 114, 3999-4004.

7.   Single-Crystalline EuF3 Hollow Hexagonal Microdisks: Synthesis and Application as a Background-Free Matrix for MALDI-TOF-MS Analysis of Small Molecules and Polyethylene Glycols. Zhiming Chen, Zhirong Geng, Dalin Shao, Yuhua Mei, Zhilin Wang*. Anal. Chem. 2009, 81, 7625-7631.

8.   Composites of Multiwalled Carbon Nanotubes and Molecularly Imprinted Polymers for Dopamine Recognition. Xianwen Kan, Yao Zhao, Zhirong Geng, Zhilin Wang *, Jun-Jie Zhu. J. Phys. Chem. C 2008, 112, 4849-4854.



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