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何卫江,教授
 

Email: heweij69@nju.edu.cn

Tel: 0086-25-83597066

何卫江 教授

Prof. weijiang He

2009-present                Professor, Nanjing University

2003-2009                    Associated Professor, Nanjing University

2001-2002                    Postdoctoral Associate in Max-Planck Institute of Colloids and Interfaces

1997-2001                    PhD in Inorganic Chemistry, Coordination Chemistry Institute, Nanjing University

1994-1997                    MSc. in Organic Chemistry, Nanjing University

1991-1994                    R&D Chemical Engineer, Jiangyin Pesticide Company

1987-1991                    BSc. in Chemistry, Nanjing University

         何卫江       教授、博士生导师。1991年于南京大学化学系有机化学专业获得学士。1991-1994年在江阴农药厂研究所从事农药合成研发工作。1997年于南京大学化学化工学院有机化学专业获得硕士学位。2001年在南京大学配位化学国家重点实验室获得博士学位。随后在德国马普胶体与界面研究从事博士后研究。2003年其在南京大学配位化学研究所工作,2009年晋升为教授。研究方向为生物相关无机小分子物种的荧光探针设计、造影应用以及这些小分子的生物无机化学;光活化金属配合物与生物分子的相互作用。

Research Interests

Fluorescence imaging of signaling molecules Zn2+ and H2S

Zinc plays essential roles in signal transduction, neurotransmission, and gene transcription etc. Development of Zn2+ sensors to realize the subcellular Zn2+ imaging in specific organelles and the in vivo Zn2+ imaging in animal mode is our current interest, with the purpose to promote the understanding and theranostic application of Zn2+ biology. With the Zn2+ sensor developed in our lab, NBD-TPEA, the first in vivo Zn2+ imaging has been realized in live zebrafish larva, and the in vivo Zn2+ imaging in mammalian animal mode with the improved Zn2+ sensor is still undergoing. In addition, the subcellular Zn2+ imaging in mitochondria and nucleus with Zn2+ sensor of specific organelle-targetability has also been realized.

H2S was recognized as the third endogenous gasotransmitter after NO and CO. It can induce vasodilation, and displays the function of antioxidation, anti-apoptosis and anti-inflammation. The development of H2S probes of rapid response to realize the real time H2S tracking is especially required to acquire the real time information of endogenous H2S. A ratiometric H2S probe CouMC of quick response (<30 s) was prepared and the tracking of quick H2S enhancement in mitochondria (<80 s) upon NaHS incubation was realized with this probe.

Fluorescent tracking of paramagnetic metals in living cells

Overcoming the paramagnetic emission quenching effect to realize the in situ fluorescence imaging of Cu2+ and Fe3+ is challenging, and the electronic decoupling of metal center from fluorophore and reducing the metal paramagnetism with elaborately designed chelator are the reliable design rationales. Two ratiometric Cu2+ fluorescent sensors have been developed in our lab, and the ratiometric intracellular Cu2+ imaging has been realized. In addition, several Fe3+ sensors of turn-on response have been constructed with different sensing mechanism, and their reversible sensing ability over other reported Fe3+ sensors provides them the ability to track Fe3+ fluctuation in cells.

Tracking the exogenous toxic metal cations and metal-based drugs

Exogenous bioinorganic species are also closely associated with health. For example, Cd2+ and Hg2+ will lead to the irreversible damage to endocrine and nervous systems of human body. Overcoming the Zn2+ interference and the emission quenching effect of heavy atoms is the main task to construct fluorescent probes for Cd2+ and Hg2+ and realize the in vivo tracking and clarify their toxicology. The high sensitivity is another key point for these probes. High sensitive Hg2+ probe DT-ABD was designed based on the Hg2+-induced carbaldehyde recovery from 1,3-dithiane. With the detection limit of 4 nM (US EPA standard for drinking water 10 nM), the in vivo Hg2+ tracking in Hg2+-polluted zebrafish larva was realized using this probe. In addition, a ratiometric Cd2+ sensor DBITA of detection limit ~0.3 pM (US EPS standard for drinking water 40 nM) was developed, and its intracellular Cd2+ imaging ability was confirmed.

The cell and in vivo uptake, distribution and metabolism of metal-based anticancer agents are especially helpful to understand and improve their antitumor activity. Modifying these metal complexes with fluorophore has been confirmed to be a reliable means to acquire the above mentioned information via fluorescence imaging. With this method, the uptake, distribution of the chelating monofunctional Pt(II) complex in living cells and zebrafish larva have been investigated.

Selected Publications

1.     A reversible ratiometric sensor for intracellular Cu2+ imaging: metal coordination-altered FRET in a dual fluorophore hybrid, Yuncong Chen, Chengcheng Zhu, Jiajie Cen, Jing Li, Weijiang He,* Yang Jiao and Zijian Guo*, Chem. Commun. 2013, 49, 7632—7634.

2.     Metal coordination in photoluminescent sensing, Zhipeng Liu, Weijiang He*and Zijian Guo*, Chem. Soc. Rev. 2013, 42, 1568-1600.

3.     A ratiometric fluorescent probe for rapid detection of hydrogen sulfide in mitochondria, Yuncong Chen, Chengcheng Zhu, Zhenghao Yang, Junjie Chen, Yafeng He, Yang Jiao, Weijiang He,* Lin Qiu, Jiajie Cen and Zijian Guo*, Angew. Chem. Int. Ed. 2013, 52, 1688 -1691.

4.     A new ‘‘turn-on’’ chemodosimeter for Hg2+: ICT fluorophore formation via Hg2+-induced carbaldehyde recovery from 1,3-dithiane, Yuncong Chen, Chengcheng Zhu, Zhenghao Yang, Jing Li, Yang Jiao, Weijiang He*, Junjie Chen and Zijian Guo*, Chem. Commun. 2012, 48, 5094–5096.

5.     An excitation ratiometric Zn2+ sensor with mitochondria-targetability for monitoring of mitochondrial Zn2+ release upon different stimulations, Zhipeng Liu, Changli Zhang, Yuncong Chen, Weijiang He* and Zijian Guo*, Chem. Commun. 2012, 48, 8365–8367.

6.     A highly sensitive ratiometric fluorescent probe for Cd2+ detection in aqueous solution and living cells, Zhipeng Liu, Changli Zhang, Weijiang He,* Zhenghao Yang, Xiang Gao and Zijian Guo*, Chem. Commun. 2010, 46, 6138–6140.

7.     A Zn2+ fluorescent sensor derived from 2-(pyridin-2-yl)benzoimidazole with ratiometric sensing potential, Zhipeng Liu, Changli Zhang, Yunling Li, Zhengyi Wu, Fang Qian, Xiaoliang Yang, Weijiang He,* Xiang Gao and Zijian Guo*, Org. Lett. 2009, 11,795-798.

8.       Visible light excitable Zn2+ fluorescent sensor derived from an intramolecular charge transfer fluorophore and its in vitro and in vivo application, Fang Qian, Changli Zhang, Yumin Zhang, Weijiang He,* Xiang Gao, Ping Hu and Zijian Guo*, J. Am. Chem. Soc. 2009, 131, 1460-1468.



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